janna
Moderator
Hair restoration is an ever changing field and clinics have to keep up with the times by learning and sharing new techniques, instrumentations, and protocol with other physicians in this industry. Dr. Jerry Cooley is the physician who put Shapiro Medical onto Hypo Thermosol solution back in 2009. We've been using Hypo Thermosol from Biolife Solutions since February of 2010 so over 3 years already (oh, how time flies!). It is more expensive over the widely used saline, however, we want to maximize yield whenever and wherever possible. We didn't know how helpful it would be but we knew it wouldn't do any harm. After all, Dr. Cooley felt it was worth while. After 3 years, we too believe we get better yield with it. Many clinics have converted to Plasma Lite over saline so it's not just a saline vs Hypo Thermosol debate. There are other holding solutions as well so it's worth checking with your clinic before undergoing your procedure.
Below is a short article written by Dr. Paul McAndrews on why he uses Hypo Thermosol holding solution at his clinic.
The informative article was sent to us by an inquiring forum poster on another site. I thought members on here may find it of interest.
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BioLife s HypoThermosol is increasingly being used by leading hair transplant physicians as an optimized ex-vivo holding solution for both the explanted tissue strip and dissected hair follicles.
The formulation of HypoThermosol has been shown to extend the ex-vivo storage interval for these biologics, while maintaining viability and in some cases, promoting faster engraftment of transplanted follicles.
A series of comparative experiments on HypoThermosol and the standard saline solution were conducted by the research team at BioLife Solutions.
Figure 1 below includes representative flourescent micrographs (A, B) and brightfield images (C, D) illustrating the morphology of hair follicles stored in HypoThermosol or saline.
Figure 2 is a graph of follicle viability after extended ex-vivo storage and extended culture conditions in either storage solution.
Figure 3 illustrates six month hair re-growth for follicles stored in either solution (different follicles used in Figures 1 and 3, Figure 3 image provided by William Parsley, M.D
The most significant limitation to hair transplantations is that there is a fixed and limited amount of good genetic hair that can be transferred to the balding area. Considering this limitation, physicians should use techniques and technologies that ensure the highest percent of grafts survive and grow.
This is why I use HypoThermosol as the holding solution for the grafts.
HypoThermosol reduces the harmful effects of tissue reperfusion injury by:
scavenging free radicals
preventing gradient formation that causes shrinking and swelling in response to temperature changes
minimizing apoptosis and necrosis by maintaining proper pH, structural integrity, and ionic balance inside and outside the cell membrane
Below is a short article written by Dr. Paul McAndrews on why he uses Hypo Thermosol holding solution at his clinic.
The informative article was sent to us by an inquiring forum poster on another site. I thought members on here may find it of interest.
**********************************************************
BioLife s HypoThermosol is increasingly being used by leading hair transplant physicians as an optimized ex-vivo holding solution for both the explanted tissue strip and dissected hair follicles.
The formulation of HypoThermosol has been shown to extend the ex-vivo storage interval for these biologics, while maintaining viability and in some cases, promoting faster engraftment of transplanted follicles.
A series of comparative experiments on HypoThermosol and the standard saline solution were conducted by the research team at BioLife Solutions.
Figure 1 below includes representative flourescent micrographs (A, B) and brightfield images (C, D) illustrating the morphology of hair follicles stored in HypoThermosol or saline.
Figure 2 is a graph of follicle viability after extended ex-vivo storage and extended culture conditions in either storage solution.
Figure 3 illustrates six month hair re-growth for follicles stored in either solution (different follicles used in Figures 1 and 3, Figure 3 image provided by William Parsley, M.D
The most significant limitation to hair transplantations is that there is a fixed and limited amount of good genetic hair that can be transferred to the balding area. Considering this limitation, physicians should use techniques and technologies that ensure the highest percent of grafts survive and grow.
This is why I use HypoThermosol as the holding solution for the grafts.
HypoThermosol reduces the harmful effects of tissue reperfusion injury by:
scavenging free radicals
preventing gradient formation that causes shrinking and swelling in response to temperature changes
minimizing apoptosis and necrosis by maintaining proper pH, structural integrity, and ionic balance inside and outside the cell membrane
